Safoura Ghalamkari
University of Debrecen, Hungary
Title: A novel carcinogenic PI3Kα mutation suggesting the role of helical domain in transmitting nSH2 regulatory signals to kinase domain
Biography
Biography: Safoura Ghalamkari
Abstract
Mutations in PIK3CA, which encodes p110α subunit of PI3K class IA enzymes are highly frequent (~40%) in breast cancer. Most of the hotspot gain-of-function PIK3CA mutations are clustered in exon 9 and 20 of this gene. In this study, we aimed to probe mutations in exon 9 of PIK3CA and computationally simulate their function since MD simulation is a powerful tool for predicting conformational dynamics of mutant protein kinases. Firstly PCR/HRM and PCR/sequencing were used for mutation detection in 40 breast cancer specimens. The identified mutations were queried via in-silico algorithms to check the pathogenicity.